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dagr.tasks.picard

FastqToUnmappedSam

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object FastqToUnmappedSam

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  4. def apply(fq1: PathToFastq, fq2: PathToFastq, bam: PathToBam, sm: String, lb: String, pu: String, rgId: Option[String] = None, prefix: Option[Path], qualityFormat: Option[FastqQualityFormat] = None): Pipe[Nothing, SamOrBam]

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    Constructs a new set of piped tasks for taking PE Illumina data from two fastq files and producing an unampped BAM file in which the possible location of adapter sequence in each read is marked.

    Constructs a new set of piped tasks for taking PE Illumina data from two fastq files and producing an unampped BAM file in which the possible location of adapter sequence in each read is marked.

    fq1

    the read one fastq file (can be gzipped)

    fq2

    the read two fastq file (can be gzipped)

    bam

    the output SAM or BAM file to write to

    sm

    the name of the sample to put in the read group header

    lb

    the name of the library to put in the read group header

    pu

    the platform unit string to put in the tread group header

    rgId

    the optional ID to use in the read group header. If None one will be generated.

    prefix

    the prefix (including directories) to use to write metrics and ancillary files

    returns

    a Pipe from Nothing (since the inputs are read from file) to SamOrBam

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